This test consists of the Large Volume Barile Method (M-300) coupled with the indicator cell culture assay. Advantages of this combination approach include detection of both the cultivable and non-cultivable mycoplasma species.
Citation:
Barile, M. F. and Kern, J. Isolation of Mycoplasma arginini from Commercial Bovine Sera and Its Implication in Contaminated Cell Cultures. Proc. Soc. Exp. Biol. 138:432-437 (1971).
Total Testing Time:
28 days
Sample Requirements:
Minimum Test Volume = 90 mL
100 mL aliquot preferred
The M-350 Mycoplasma Test is a highly sensitive and reliable method for the detection of mycoplasma contamination in fetal bovine serum, culture media, and allied materials used to generate biopharmaceutical products.
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