CELLshipper® Mycoplasma Detection Kit

M-100 Data Sheet

Our exclusive CELLshipper® DNA fluorochrome staining kit is a fast and convenient qualitative assay for in situ detection of Mycoplasma contamination in cell cultures. Purchase the slides and store in your lab for use at your convenience. Simply add your cell culture sample, fix and dry. Return the (hazard-free) slides to our laboratory for DNA fluorochrome staining and diagnosis.

Intended Use:

Our CELLshipper® kit is a direct DNA fluorochrome staining kit specifically developed by Bionique Testing Laboratories, Inc. for the detection of mycoplasma contamination in cell cultures.

Description:

This test kit offers a convenient and efficient method for screening a variety of cell culture samples (adherent and non-adherent cultures). The sensitivity of this assay for the detection of mycoplasma is ≥ 95%, as verified by numerous studies*. Purchase the slides and store them in your lab for use at your convenience. You simply add your cell culture sample, incubate and fix the slide within the fixation/staining chamber following the instructions provided. Following fixation, the slide is returned to our laboratory for final processing and diagnostic evaluation. Positive and negative quality controls are processed and analyzed with each sample batch.

 Bionique Testing Laboratories will provide results within 48 hour of receipt of your CELLshipper® slide.

Kit Components:

  • Four (4) CELLshipper® slides specially treated to enhance cell attachment
  • Four (4) fixation and staining slide chambers
  • Four (4) pre-addressed padded mailers
  • Protocol

Procedure:

Refer to CELLshipper® Instructions or Product Insert for directions.

Storage/Handling:

Store at room temperature.

Packaging:

Each order contains four (4) individual test kits.

For Research Use Only. Not for clinical diagnostic purposes.

CELLshipper® Mycoplasma Detection Kit
Cat. # M-100: 4 individual test kits/Pkg. or 40 individual test kits/Case
For additional product support or information visit www.bionique.com or contact us at (518) 891-2356.

*References:

  1. Chen, TR. 1977. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoeschst 33258 stain. Exp. Cell Res. 104: 255-262.
  2. Del Guidice, RA, Hopps, HS. 1977. Microbiological methods and fluorescent microscopy for direct demonstration of mycoplasmal infection in cell cultures. In Mycoplasma Infection of Cell Cultures (McGarrity, Murphy and Nichols, eds.), Plenum Press, New York pp. 57-69.
  3. Lincoln, CK, Gabridge, MG. 1998. Cell culture contamination: Sources, consequences, prevention and elimination. Methods in Cellbiology, vol. 57 Chapter 4, pp. 49-65. Academic Press, New York.
  4.  McGarrity, GJ, Steiner, T, Vanaman, V. 1983. Detection of mycoplasmal infection of cell cultures by DNA fluorochrome staining. In Methods of Mycoplasmology, Vol. II (Tully and Razin, eds.) Academc Press, New York, pp. 183-190.
  5. McGarrity, GJ, Sarama, J. and Vanaman, V. 1979. Factors influencing microbiological detection in cell cultures. In Vitro. 15:73-81.
Shipping Instructions:

Return slides by First Class U.S. Mail. For faster service use overnight courier.



Sample Submission Form
FOR INFORMATION, CALL
$220/Pkg. (4 Individual Test Kits)
$1,870/Case (40 Individual Test Kits)
Total Testing Time: Results within 48 hours of receipt.